Gynecology, Obstetrics and Perinatology

Proteins, cytokines and antigen-specific immunocomplexes of follicular fluid and blood serum in women in in-vitro fertilisation

In follicular fluid (FF) and blood serum of 45 women with infertility of tubular genesis who entered the program of in-vitro fertilisation and underwent induction of superovulation, protein concentrations (total protein, albumin, alpha-2-macroglobulin, IgG,  and  lactoferrin),  cytokines  (TNF-?,  IFN-?,  IL-6,  and  IL-1r),  and  also  antigen-specific  immune  complexes  consisting  of alpha-2-macroglobulin and IgG (MG–IgG), lactoferrin and IgG (LF-IgG) were determined. Blood and FF were taken during transvaginal punction of the follicules performed after triggering of ovulation. Serum of 40 practically healthy non-pregnant women was used for control. In women diagnosed with «infertility of tubular genesis» in unsuccessful programs (IVF-) low levels of IL-6 and IL-1r in follicular fluid were detected along with unchanging concentrations of proteins (MG, LF, albumin and total protein), IgG antibodies, immune complexes (MG–IgG and LF–IgG) and cytokines (TNF-? and IFN-?). Higher serum concentrations of the immunocomplexes MG–IgG, IFN-? and TNF-? were determined in the group of patients with the negative result (IVF-) as compared with evidence in women from the group with the positive result (IVF+) and the indices in the group of healthy women. The results might be used as prognostic criteria when carrying out the program of in-vitro fertilisation in women with infertility of tubular genesis.